We are happy to provide the following selection of clinical references on Provocholine:

Resource Menu International Insert MSDS Dilution Guidelines References

Dilution Sequences

Methapharm is happy to provide healthcare practitioners with the following instructional aides for common dilution sequences:


Dilution Sequence Protocol: ATS Long (Single Patient)
Dilutions: 0.031 mg/mL, 0.0625 mg/mL, 0.125 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1 mg/mL, 2 mg/mL, 4 mg/mL, 8 mg/mL, 16 mg/mL

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Dilution Sequence Protocol: ATS Five-Breath Dosimeter Method (Single Patient)
Dilutions: 0.0625mg/mL, 0.25 mg/mL, 1 mg/mL, 4 mg/mL, 16 mg/mL

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Package Insert Provocholine (methacholine chloride USP)


Canadian Package Insert for Provocholine
(methacholine chloride USP)

The above Prescribing Information in intended Canadian Healthcare Professionals. The product discussed herein may have different product labeling in different countries. For the prescribing information related to a specific country, please contact Methapharm.

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Material Safety Data Sheet (MSDS)


MSDS for Provocholine - English (PDF)

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We are happy to provide links to the following guidelines that address the use of methacholine challenge testing:

American Thoracic Society Guidelines for Methacholine and Exercise Challenge Testing - 1999 (Published in American Journal of Respiratory & Critical Care Medicine, Vol. 161:1, January 2000 pp. 309-329)

The National Heart, Lung, and Blood Institute (NHLBI)

AARC Clinical Practice Guidelines Methacholine Challenge Testing 2001 Revision & Update

The ACCP Clinical Practice Guidelines Diagnosis and Management of Cough (Published in CHEST Journal 129.1, January 2006 Supplement (pp. 222 - 231).

"In patients whose chronic cough persists after treatment for UACS, the possibility that asthma is the cause of cough should be worked up next. The medical history is sometimes suggestive, but is not reliable in either ruling in or ruling out asthma. Therefore, ideally, BPC, if spirometry does not indicate reversible airflow obstruction, should be performed in the evaluation for asthma as a cause of cough."

Chronic Cough Due to Asthma ACCP Evidence-Based Clinical Practice Guidelines Peter V. Dicpinigaitis, MD, FCCP, 10.1378/chest.129.1_suppl.75S CHEST January 2006 vol. 129 no.1 suppl 75S-79S

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We are happy to provide the following selection of clinical references on Provocholine:


Bronchial Provocation with Methacholine

A Literature Review of Safety by TechEd

Cleveland Clinic January 2008 - 1 Minute Consult "When should a methacholine challenge be ordered for a patient with suspected asthma?"

The Childhood Asthma Management Program Research Group (CAMP) Article on safety of MCT
Long-Term Effects of Budesonide or Nedocromil in Children with Asthma The New England Journal of Medicine Volume 343:1054-1063 October 12, 2000 Number 15

The Science Journal of the American Association for Respiratory Care Clinical Variables Are Poor Selection Criteria for the Use of Methacholine Bronchoprovocation in Symptomatic Subjects Annie Lin Parker MD and Muhanned Abu-Hijleh MD, Respir Care. 2003 Jun;48(6):596-601.

The Science Journal of the American Association for Respiratory Care Irvin C. Bronchial challenge Just do it! Resp Care 2003; 48:589-590.

CHEST An Empiric Integrative Approach to the Management of Cough ACCP Evidence-Based Clinical Practice Guidelines 10.1378/chest.129.1_suppl.222S CHEST January 2006 vol. 129 no. 1 suppl 222S-231S

Journal of Asthma 2004-ECT vs. MCT-Brown Madigan Army Medical Center Airway Hyperresponsiveness by Methacholine Challenge Testing Following Negative Exercise Challenge Linda L. Brown; Bryan L. Martin; Michael J. Morris Journal of Asthma, 1532-4303, Volume 41, Issue 5, 2004, Pages 553 – 558

*Please note that this publication is only available with a fee.

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While we use reasonable efforts to include accurate and up-to-date information, we make no warranties or representation with respect to the accuracy, currency, or completeness of any of the materials. Please contact us if you would like any further information or to report any discrepancies you may find.