The methacholine challenge test was first approved by the FDA in 1986 and since then has been included in various asthma, and bronchial challenge testing guidelines (both international and country specific).


Please find below links to some examples of relevant guidelines/recommendations which include methacholine challenge testing;

ERS technical standard on bronchial challenge testing: general considerations and performance of methacholine challenge tests

Coates AL, Wanger J, Cockcroft DW, et al.
EurRespir J 2017; 49: 1601526 [].

Guidelines for Methacholine and Exercise Challenge Testing—1999

Crapo RO, Casaburi R, Coates AL, et al.
Am J RespirCrit Care Med 2000; 161: 309–329